Harvard University, Cambridge, Massachusetts, 2004-present.
Postdoctoral Fellow in the lab of Professor Raymond Erikson, 2004-present.
Research designed to elucidate the signal transduction pathways and roles of polo-like kinases in the mammalian cell cycle.  Current project “The Function of Plk3.” Identified Polo-like kinase 3 as an essential regulator of the G1-S phase transition of the cell cycle, and as a regulator of abscission.    Identified cyclin E as a down-stream target of Polo-like kinase 3.

University of Massachusetts Medical Center, Worcester, MA 1996-2004
Research Associate in the lab of Stephen Doxsey. 1996-2004.
Investigated the role of the centrosomal protein pericentrin in centrosome structure and spindle assembly. Identified pericentrin as a minus-end anchor for microtubules in the vertebrate spindle.

EcoScience Corporation, Worcester, Massachusetts
Associate Scientist - Process Development, 1992-1995.
Responsible for development and scale up of Fungal Production Systems, developed novel solid -state and liquid systems for production of specific fungal propagules and integration of those systems with conventional fermentation methods. Developed and selected fungal strains for use in production. Trained and supervised junior employees. Participated on product development teams.

Repligen Sandoz Research Corporation, Lexington, Massachusetts
Associate Scientist – Discovery-Pulp Improvement Project and MBI, 1991-1992
Research Associate –Discovery- Pulp Improvement Project and Biocatalyzed Projects, 1988-1991.
Responsible for Direction and coordination of two unrelated projects involving joint studies between: RSRC, MBI, academic researchers, and technical support personnel.  Performed feasibility studies and development of assays employing enzymes and synthetic biomimetic catalysts in normal and multiphase environments. Developed a novel enzymatic process (employing a multi-phasic system) for synthesis of an industrially important chemical from industrial waste material. Produced, purified and characterized isozymes for use in novel industrial applications. Developed new methods leading to the identification of fungi effective in reduction of pitch from wood (CartapipTM).  Developed methods needed to produce industrially useful fungal strains and produced patented fungal strains with proven effectiveness under industrial conditions. Identified of a number of physiological characteristics necessary/ responsible for effective growth of CartapipTM in the field.  Conducted field trials, trained Sandoz technical support personnel, and supported Sandoz sales personnel. Prepared technical reports and summaries. Produced and maintained a large culture collection.

Scientific skills
Extensive Cell Biology, Biochemistry, and Molecular Biology expertise including:
• Mammalian tissue culture and transfection.
• Bacterial, insect, fungal, and mammalian cell line protein expression,
• Purification and functional analysis of proteins including, sub-cellular fractionation, immunoprecipitation, ultracentrifugation, sucrose gradients, westerns, SDS-PAGE, FPLC and HPLC.
• Development of enzyme assays.
• RNA/ DNA extraction, RT-PCR, cloning.
• RNAi technology, including siRNA, shRNAi and lentivirus based RNAi.
• Production of lentivirus.
• Fluorescence, confocal, phase and live imaging microscopy.